PoplarV1, Main, Exploration, bibRecord, 003149

Quantitative analysis of cytokinins in plants by high performance liquid chromatography: electronspray ionization ion trap mass spectrometry.

Identifieur interne : 003149 ( Main/Exploration ); précédent : 003148; suivant : 003150

Quantitative analysis of cytokinins in plants by high performance liquid chromatography: electronspray ionization ion trap mass spectrometry.

Auteurs : Weiqi Chen [République populaire de Chine] ; Ying Gai ; Shichang Liu ; Renxiao Wang ; Xiangning Jiang

Source :

Journal of integrative plant biology [ 1744-7909 ] ; 2010.

RBID : pubmed:20883444

Descripteurs français

KwdFr :
- Chromatographie en phase liquide à haute performance (méthodes), Cytokinine (analyse), Cytokinine (composition chimique), Feuilles de plante (composition chimique), Oryza (composition chimique), Parties aériennes de plante (composition chimique), Plantes (composition chimique), Populus (composition chimique), Spectrométrie de masse ESI (méthodes).
MESH :
- analyse : Cytokinine.
- composition chimique : Cytokinine, Feuilles de plante, Oryza, Parties aériennes de plante, Plantes, Populus.
- méthodes : Chromatographie en phase liquide à haute performance, Spectrométrie de masse ESI.

English descriptors

KwdEn :
- Chromatography, High Pressure Liquid (methods), Cytokinins (analysis), Cytokinins (chemistry), Oryza (chemistry), Plant Components, Aerial (chemistry), Plant Leaves (chemistry), Plants (chemistry), Populus (chemistry), Spectrometry, Mass, Electrospray Ionization (methods).
MESH :
- chemical , analysis : Cytokinins.
- chemical , chemistry : Cytokinins.
- chemistry : Oryza, Plant Components, Aerial, Plant Leaves, Plants, Populus.
- methods : Chromatography, High Pressure Liquid, Spectrometry, Mass, Electrospray Ionization.

Abstract

The present paper introduces a highly sensitive and selective method for simultaneous quantification of 12 cytokinins (free form and their conjugates). The method includes a protocol of extraction with methanol/water/formic acid (15/4/1, v/v/v) to the micro-scale samples, pre-purification with solid phase extraction (SPE) cartridges of the extracts, separation with a high performance liquid chromatography (HPLC) and detection by an electrospray ionization ion trap mass spectrometry (ESI-Ion trap-MS) system in a consecutive ion monitoring (CRM) mode at the three stage fragmentation of mass spectrometry (MS(3) ). The lowest detection level of the cytokinins of the method reaches 0.1-2.0 pg with a very wide range of linear regression from 1-512 pg, at the coefficient factors of 0.98-0.99. The feasibility of this method has been proven in the application of the method to the analysis of the trace-amount contents of cytokinins in the micro-scale samples of various types of plant materials, such as aerial parts of rice and poplar leaves etc. 12 endogenous cytokinins had been identified and quantified in the plant tissues, with an acceptable relatively higher recovery rate from 40% to 70%.

DOI: 10.1111/j.1744-7909.2010.00989.x
PubMed: 20883444

Affiliations:

République populaire de Chine

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Le document en format XML

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<author><name sortKey="Liu, Shichang" sort="Liu, Shichang" uniqKey="Liu S" first="Shichang" last="Liu">Shichang Liu</name>
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<author><name sortKey="Wang, Renxiao" sort="Wang, Renxiao" uniqKey="Wang R" first="Renxiao" last="Wang">Renxiao Wang</name>
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<term>Populus (chemistry)</term>
<term>Spectrometry, Mass, Electrospray Ionization (methods)</term>
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<term>Cytokinine (composition chimique)</term>
<term>Feuilles de plante (composition chimique)</term>
<term>Oryza (composition chimique)</term>
<term>Parties aériennes de plante (composition chimique)</term>
<term>Plantes (composition chimique)</term>
<term>Populus (composition chimique)</term>
<term>Spectrométrie de masse ESI (méthodes)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Cytokinins</term>
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<term>Plant Components, Aerial</term>
<term>Plant Leaves</term>
<term>Plants</term>
<term>Populus</term>
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<front><div type="abstract" xml:lang="en">The present paper introduces a highly sensitive and selective method for simultaneous quantification of 12 cytokinins (free form and their conjugates). The method includes a protocol of extraction with methanol/water/formic acid (15/4/1, v/v/v) to the micro-scale samples, pre-purification with solid phase extraction (SPE) cartridges of the extracts, separation with a high performance liquid chromatography (HPLC) and detection by an electrospray ionization ion trap mass spectrometry (ESI-Ion trap-MS) system in a consecutive ion monitoring (CRM) mode at the three stage fragmentation of mass spectrometry (MS(3) ). The lowest detection level of the cytokinins of the method reaches 0.1-2.0 pg with a very wide range of linear regression from 1-512 pg, at the coefficient factors of 0.98-0.99. The feasibility of this method has been proven in the application of the method to the analysis of the trace-amount contents of cytokinins in the micro-scale samples of various types of plant materials, such as aerial parts of rice and poplar leaves etc. 12 endogenous cytokinins had been identified and quantified in the plant tissues, with an acceptable relatively higher recovery rate from 40% to 70%.</div>
</front>
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Quantitative analysis of cytokinins in plants by high performance liquid chromatography: electronspray ionization ion trap mass spectrometry.

Quantitative analysis of cytokinins in plants by high performance liquid chromatography: electronspray ionization ion trap mass spectrometry.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki